Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Klink, Vincent
Committee Member
Gordon, Donna M.
Committee Member
Wang, Ying
Committee Member
Li, Jiaxu
Date of Degree
4-30-2021
Original embargo terms
Complete embargo for 2 years
Document Type
Graduate Thesis - Open Access
Major
Biology
Degree Name
Master of Science
Degree Name
Master of Science (M.S.)
College
College of Arts and Sciences
College
College of Arts and Sciences
Department
Department of Biological Sciences
Department
Department of Biological Sciences
Abstract
The histone 2A (H2A) variant Z (H2A.Z) regulates gene expression, replacing H2A predominately at +1 nucleosomes. Whereas, H2A.Z can act as either a positive or negative transcriptional regulator, this research focuses on its transcriptional activation role. This thesis focuses on examining the Glycine max HTA9 gene family (H2A.Z9). The Arabidopsis thaliana HTA9 protein sequence was used to query the G. max proteome resulting in identification of five H2A.Z9 (H2A.Z9-1-5) paralogs. H2A.Z9-1, H2A.Z9-2, H2A.Z9-4, and H2A.Z9-5 are expressed within Heterodera glycines-parasitized root cells undergoing defense. All 5 paralogs, including H2A.Z9-3, were studied in transgenic-functional analyses. Data demonstrates that H2A.Z9 overexpression leads to a 60-70% reduction in H. glycines- parasitism with no effect on root growth. In contrast, H2A.Z9 knockdown by RNAi results in a 3.5-5.0-fold increase in H. glycines-parasitism with no effect on root growth. These results demonstrate that H2A.Z9 is important to G. max defense toward H. glycines-parasitism and indicate possible redundant or specific roles for each paralog.
Sponsorship
Teaching Assistantship, Department of Biological Sciences
Recommended Citation
Acharya, Sudha, "A functional analysis of the Glycine max H2A.Z9 gene family in relation to defense to Heterodera glycines parasitism" (2021). Theses and Dissertations. 5073.
https://scholarsjunction.msstate.edu/td/5073