Theses and Dissertations

Issuing Body

Mississippi State University

Advisor

Dongmao Zhang

Committee Member

Todd E. Mlsna

Committee Member

Joseph P. Emerson

Date of Degree

8-6-2021

Original embargo terms

Complete embargo for 1 year

Document Type

Graduate Thesis - Open Access

Major

Chemistry

Degree Name

Master of Science

College

College of Arts and Sciences

Department

Department of Chemistry

Abstract

Kinetic spectroscopic quantification refers to a subset of chromogenic (CG) and fluorogenic (FG) assays that deduce analyte concentration based on the UV-vis or fluorescence signal obtained during the CG/FG reaction processes. Existing kinetic spectroscopic quantification are based predominantly on reactions that can be approximated as a first-order process. Presented in this thesis is the kinetic spectroscopic quantification that uses higher order CG/FG reactions where the overall reaction can be approximated as combination of two sequential first-order processes. Included in chapter one is the theoretical model and several proof-of-concept applications. This model analyte is malondialdehyde (MDA), a lipid peroxidation biomarker of broad interest. Chapter two describes the study of the effects of the reaction solvent, temperature, acid catalyst, and calibration method on the assay performance. The most rapid MDA assays achieved so far is 3 mins, 30 times more efficient than the current equilibrium spectroscopic quantification.

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