Theses and Dissertations

Issuing Body

Mississippi State University


Kouba, Andrew J.

Committee Member

Vance, Carrie K.

Committee Member

Morin, Dana

Date of Degree


Document Type

Graduate Thesis - Open Access


Wildlife, Fisheries and Aquaculture

Degree Name

Master of Science (M.S.)


College of Forest Resources


Department of Wildlife, Fisheries and Aquaculture


Limited breeding success in captive breeding programs has necessitated the development of assisted reproductive technologies (ART) to preserve and increase genetic variation and population numbers of both captive and wild amphibian groups. ART has been shown to be successful in numerous anuran species, and current studies focus on the application of ART in ex-situ populations. The focus of this project is to show that linking in-situ and ex-situ amphibian populations through sperm cryopreservation, genome banking, and in-vitro fertilization is possible, with the goal of increasing gene diversity throughout groups in order to produce self-sustaining, wild populations in the future. Specific objectives include developing a sperm-cryopreservation methodology using sperm from the model species Anaxyrus fowleri, applying this protocol to the cryopreservation of spermatozoa from two other threatened anurans to determine protocol transmissibility, and linking in-situ and ex-situ populations of an endangered species using cryopreserved sperm form wild males to produce viable offspring.