Theses and Dissertations

ORCID

0000-0001-7759-4722

Issuing Body

Mississippi State University

Advisor

Shanmugam, Shankar G

Committee Member

Kingery, William

Committee Member

Rushing, Brett

Committee Member

Cox, Michael S

Date of Degree

8-9-2022

Document Type

Graduate Thesis - Open Access

Major

Agronomy

Degree Name

Master of Science (M.S.)

College

College of Agriculture and Life Sciences

Department

Department of Plant and Soil Sciences

Abstract

Integrating crop and livestock is being considered to improve soil health by carbon sequestration. A two-year study (2019-2021) at CPBES in Newton, MS was aimed to evaluate soil microbial diversity in the warm, humid regions, specifically southeastern USA. Amplicons targeting bacterial 16S rRNA genes and fungal ITS2 regions were sequenced. Taxonomic assignment and microbial diversity characterization were performed using QIIME2®. Soil fungal diversity showed significant differences (alpha diversity, p = 0.031 in yr. 2020 and beta diversity, p = 0.037 in yr. 2021). Canonical Correspondence Analysis (CCA) and Mantel test showed significant influence on fungal diversity due to carbon (rm = 0.2581, p = 0.022), nitrogen (rm = 0.2921, p = 0.0165) in yr. 2021, and on bacterial diversity due to EE-GRSP (rm = 0.22, p = 0.02) in yr. 2020. Long term study of ICLS can help us better understand the shift in microbiome to improve crop production sustainably.

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