Mississippi State University
Shanmugam, Shankar G
Cox, Michael S
Date of Degree
Graduate Thesis - Open Access
Master of Science (M.S.)
College of Agriculture and Life Sciences
Department of Plant and Soil Sciences
Integrating crop and livestock is being considered to improve soil health by carbon sequestration. A two-year study (2019-2021) at CPBES in Newton, MS was aimed to evaluate soil microbial diversity in the warm, humid regions, specifically southeastern USA. Amplicons targeting bacterial 16S rRNA genes and fungal ITS2 regions were sequenced. Taxonomic assignment and microbial diversity characterization were performed using QIIME2®. Soil fungal diversity showed significant differences (alpha diversity, p = 0.031 in yr. 2020 and beta diversity, p = 0.037 in yr. 2021). Canonical Correspondence Analysis (CCA) and Mantel test showed significant influence on fungal diversity due to carbon (rm = 0.2581, p = 0.022), nitrogen (rm = 0.2921, p = 0.0165) in yr. 2021, and on bacterial diversity due to EE-GRSP (rm = 0.22, p = 0.02) in yr. 2020. Long term study of ICLS can help us better understand the shift in microbiome to improve crop production sustainably.
Sinha, Namita, "Effect of cover crops, grazing and tillage practices on soil microbial community composition, function, and soil health in east central Mississippi soybean production system." (2022). Theses and Dissertations. 5558.