Development of a crosslinked osteochondral xenograft and a collagen stabilizing intra-articular injection to remediate cartilage focal lesions to prevent osteoarthritis

Author

Mark Lewis Mosher, Mississippi State UniversityFollow

Issuing Body

Mississippi State University

Advisor

Elder, Steven H.

Committee Member

Butler, James Ryan

Committee Member

Priddy, Lauren B.

Committee Member

Simpson, C. LaShan

Committee Member

Williams, Lakiesha N.

Date of Degree

12-9-2022

Document Type

Dissertation - Open Access

Major

Biomedical Engineering

Degree Name

Doctor of Philosophy (Ph.D)

College

James Worth Bagley College of Engineering

Department

Department of Agricultural and Biological Engineering

Abstract

Osteoarthritis is one of the most common causes of disability in adults in America. It is a progressive and degenerative disease where the articular cartilage is broken down and lost from the surfaces of bones causing chronic pain and swelling in the joints, and currently has no cure. The most commonly osteoarthritis starts from a focal lesion on the cartilage surface, which will expand on the surface and downwards through the thickness of the tissue. The current gold standard for correcting cartilage focal lesions is the osteochondral autograft/allograft transplantation (OAT), which replaces the defect with a fresh osteochondral graft. The main limiting factor for using the OAT comes from the limited number of autograft and allografts that are available for implantation. To address the concern of graft availability, this study will look at the development of a porcine osteochondral xenograft (OCXG). The first aim of this research is to establish a decellularization protocol that will remove the antigens and cellular debris, which are the leading causes of graft rejection when implanting animal tissue in humans. The second aim of this study is restoring the mechanical strength of the OCXG that was lost during the decellularization process through crosslinking the tissue using genipin and epigallocatechin gallate (EGCG). The third aim is comparing the performance of the complete crosslinked OCXG at different degrees of crosslinking in a long-term goat animal model. The final aim is an alternative way to correct focal lesions through the development of an injectable collagen stabilizing treatment with genipin and punicalagin that will slow or stop the growth of a lesion and prevent osteoarthritis.

Recommended Citation

Mosher, Mark Lewis, "Development of a crosslinked osteochondral xenograft and a collagen stabilizing intra-articular injection to remediate cartilage focal lesions to prevent osteoarthritis" (2022). Theses and Dissertations. 5679.
https://scholarsjunction.msstate.edu/td/5679