Molecular characterization of seminal plasma from boars with divergent sperm quality

Author

Notsile Hleliwe Dlamini, Mississippi State UniversityFollow

ORCID

https://orcid.org/0000-0003-3644-6576

Issuing Body

Mississippi State University

Advisor

Nguekam-Feugang, Jean Magloire

Committee Member

Lemley, Caleb

Committee Member

Liao, Shengfa

Committee Member

Vance-Kouba, Carrie

Date of Degree

5-12-2023

Document Type

Graduate Thesis - Open Access

Major

Agriculture

Degree Name

Master of Science (M.S.)

College

College of Agriculture and Life Sciences

Department

Department of Animal and Dairy Sciences

Abstract

Seminal plasma (SP) is the microenvironment of spermatozoa whose composition reflects semen quality and constitutes an excellent source for detecting non-invasive biomarkers. This study characterizes good vs. poor quality semen using seminal extracellular vesicles coupled proteins and miRNA. Fresh boar semen samples were screened, centrifuged, and SP supernatants were collected for near-infrared spectroscopy (NIRS) analysis. EVs were extracted from SP (SP-EVs), characterized, and those of extremely poor or good sperm quality groups were subjected to proteomics and small RNA sequencing. Significant differences were set for P

Recommended Citation

Dlamini, Notsile Hleliwe, "Molecular characterization of seminal plasma from boars with divergent sperm quality" (2023). Theses and Dissertations. 5773.
https://scholarsjunction.msstate.edu/td/5773