Theses and Dissertations
Issuing Body
Mississippi State University
Advisor
Chang, Sam K.C.
Committee Member
Edelmann, Mariola J.
Committee Member
Sepehrifar, Mohammad
Committee Member
Cheng, Wen-Hsing
Committee Member
Haque, Zahur Z.
Date of Degree
8-14-2015
Document Type
Graduate Thesis - Open Access
Major
Food Science and Technology
Degree Name
Master of Science
College
College of Agriculture and Life Sciences
Department
Department of Food Science, Nutrition and Health Promotion
Abstract
Antioxidant-rich plant foods can inhibit starch and lipid digestion that are relevant to the management of type-II diabetes. Our objective was to compare the three phenolic substances (total phenolic, total flavonoids, and condensed tannin content) in crude, semi-purified extracts from eight types of foods (purified by XAD-7 column), five fractions (semi-purified extracts fractionated by Sephadex LH-20 column) from black legumes, and to compare their antioxidant capacities. The IC50 values of these crude extracts, semi-purified extracts and fractions against alpha-amylase, alpha-glucosidase and lipase were measured. Results showed that Fraction V from black soybean had the lowest IC50 value (0.25 mg/mL) against alpha-amylase; Fraction V from black bean had the lowest IC50 value (0.25 micro gram/mL) against alpha-glucosidase; Fraction IV of black bean had the lowest IC50 value (76 micro gram/mL) against lipase; myricetin showed the lowest IC50 value against alpha-amylase, alpha-glucosidase and lipase.
URI
https://hdl.handle.net/11668/19903
Recommended Citation
Tan, Yuqing, "A Comparative Study on Phenolic Substances in Selected Black Legumes that Inhibit Digestive Enzymes" (2015). Theses and Dissertations. 59.
https://scholarsjunction.msstate.edu/td/59
