Theses and Dissertations
ORCID
https://orcid.org/0000-0002-9787-2336
Issuing Body
Mississippi State University
Advisor
Priddy, Lauren B.
Committee Member
Priddy, Matthew W.
Committee Member
Jaffe, Michael
Committee Member
Elder, Steven H.
Date of Degree
12-8-2023
Original embargo terms
Campus Access Only 1 Year
Document Type
Graduate Thesis - Campus Access Only
Major
Biomedical Engineering
Degree Name
Master of Science (M.S.)
College
James Worth Bagley College of Engineering
Department
Department of Agricultural and Biological Engineering
Abstract
The current gold standard treatment for bone defects is autologous cancellous bone graft, which involves increased surgery time and donor site morbidity, and limited supply of bone and cells for regeneration. Bioreactors may aid in the generation of mechanically conditioned bone grafts with more cells compared to traditional grafts. However, the specific parameters of fluid flow and mechanical loading which contribute to osteogenesis and cell viability in bioreactors are not fully characterized. Here, a perfusion-compression bioreactor system was developed to study osteogenesis in porcine trabecular bone explants. Loading accuracy was over 88% across six bioreactors at a 0.1 s-1 strain rate and 20 N target force, akin to running. A flow rate of 0.2 mL/min appeared to be more favorable for cell viability than 1 mL/min. Overall, this work offers a foundation for future efforts to enhance cell viability and osteogenesis in bone explants.
Recommended Citation
Graham, Alexis Victoria, "Design, development, and validation of a perfusion-compression bioreactor to study osteogenesis in bone explants" (2023). Theses and Dissertations. 6016.
https://scholarsjunction.msstate.edu/td/6016