Theses and Dissertations
Advisor
Li, Ling
Committee Member
Li, Jiaxu
Committee Member
Gordon, Donna
Date of Degree
5-16-2025
Original embargo terms
Embargo 2 years
Document Type
Graduate Thesis - Open Access
Major
Biological Sciences
Degree Name
Master of Science (M.S.)
College
College of Arts and Sciences
Department
Department of Biological Sciences
Abstract
Enhancing soybean seed protein is essential for improving crop nutritional quality and addressing global food security challenges. In this study, the molecular mechanisms underlying protein enhancement through the Arabidopsis orphan gene QQS were investigated. Comparative RNA sequencing of Arabidopsis and soybean wild-type and QQS overexpression lines identified candidate genes associated with increased protein accumulation and concurrent reductions in starch deposition. Functional validation in Arabidopsis confirmed that these genes influence carbon and nitrogen allocation, making them promising targets for genetic improvement in soybean. In parallel, the impact of four commercial soil substrates on the growth of Arabidopsis thaliana and Nicotiana tabacum were assessed under controlled conditions. While soil type did not significantly affect germination, a nutrient-rich potting mix notably enhanced biomass, stem elongation, and leaf production. Together, our findings highlight the potential of integrating molecular and agronomic approaches enhance crop quality and productivity.
Recommended Citation
Brister, Ethan, "Enhancing soybean seed protein content via Arabidopsis QQS gene, and commercial soil influence on plant development" (2025). Theses and Dissertations. 6465.
https://scholarsjunction.msstate.edu/td/6465
