Theses and Dissertations

ORCID

https://orcid.org/0000-0002-3402-7725

Advisor

Renninger, Heidi

Committee Member

Pudzianowska, Marta

Committee Member

Gardiner, Emile

Committee Member

Galeano, Esteban

Date of Degree

12-12-2025

Original embargo terms

Immediate Worldwide Access

Document Type

Graduate Thesis - Open Access

Major

Forestry

Degree Name

Master of Science (M.S.)

College

College of Forest Resources

Department

Department of Forestry

Abstract

The thesis investigates the genetic structure and diversity of the Populus deltoides collection from the USDA Forest Service breeding program. Using genomics techniques, six population groups with varying levels of admixture were identified, revealing extensive historical recombination and complexity in the population structure, which contrasts with earlier studies from different regions. The research also confirms the collection's integrity by detecting operational errors and contamination, addressed through genetic identity and parentage analysis. Notably, over 80% of the clones have contributions from multiple ancestors, indicating widespread interbreeding. Additionally, a reliable database was created, covering about 96% of the unique genotypes. This enables comparisons with other collections and enhances confidence in the current set. The study provides vital resources for management, breeding, and conservation, forming a foundation for future genetic diversity assessments. Overall, it emphasizes the importance of molecular markers in analyzing genetic structure, maintaining germplasm quality, and supporting sustainable breeding practices.

Sponsorship (Optional)

This thesis is supported by the USDA Forest Service Southern Research Station, Center for Bottomland Hardwoods Research in Stoneville, Mississippi, under project 22-JV-11330170-089: Climate Smart Carbon Sequestration Resources for Bottomland Hardwood Ecosystems

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