Theses and Dissertations

ORCID

https://orcid.org/0000-0003-0539-1832

Advisor

Ganapathi, Shankar Shanmugam

Committee Member

Dhillon, Jagmandeep

Committee Member

Jordan, Heather

Committee Member

Rai, Aswathy N,

Date of Degree

12-12-2025

Original embargo terms

Immediate Worldwide Access

Document Type

Graduate Thesis - Open Access

Major

Plant and Soil Sciences (Agronomy)

Degree Name

Master of Science (M.S.)

College

College of Agriculture and Life Sciences

Department

Department of Plant and Soil Sciences

Abstract

An increasing global food demand and the urgent need for sustainable agricultural practices have driven interest in insects as alternative feed and food sources. Insect frass, a by-product of insect rearing, represents a rich organic carbon source with diverse microbes that can enhance soil health and promote beneficial plant-microbe interactions. This study characterized the bacterial (16S-V4) and fungal (ITS2) microbiomes of frass from Black Soldier Fly (BSF) and Cricket species and evaluated their effects on maize (Zea mays) growth. Four frass variants were applied at rates of 56-280 kg ha⁻¹ in a pot experiment with untreated soil and mineral fertilizer as controls. Overall, the Cricket frass showed the highest α diversity, while Firmicutes dominated BSF frass. Insect frass treatments improved nitrogen uptake and reduced soil C/N, indicating enhanced mineralization. Overall, insect frass shows strong potential as a sustainable biofertilizer, though application standardization is needed.

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