Theses and Dissertations

Advisor

Priddy, Lauren B.

Committee Member

Priddy, Matthew W.

Committee Member

Elder, Steven H.

Committee Member

Jaffe, Michael

Date of Degree

12-12-2025

Original embargo terms

Visible MSU Only 1 year

Document Type

Graduate Thesis - Campus Access Only

Major

Biomedical Engineering

Degree Name

Master of Science (M.S.)

College

James Worth Bagley College of Engineering

Department

Department of Agricultural and Biological Engineering

Abstract

Scaffolds are essential in tissue engineering, providing a 3D structure that supports cell attachment, proliferation, and differentiation while guiding tissue regeneration. Enhancing cell scaffold interactions and replicating the native mechanical and nutrient environment remain major challenges. This study integrates surface functionalization and dynamic seeding to enhance cell function on 3D printed polylactic acid (PLA) scaffolds. A custom oscillation seeding device, automated via Python, enabled bidirectional flow of cell suspension. PLA scaffolds were coated with a hybrid polydopamine/nano-hydroxyapatite coating to enhance attachment and bioactivity. Pre-osteoblasts were seeded onto coated or noncoated scaffolds either statically or using oscillation seeding, and attachment and distribution were measured after 24 hours. After seeding, scaffolds were cultured for 14 days in a bioreactor system which applied both compressive loading and perfusion flow. The study investigated the effects of bioactive coatings, oscillation seeding, and mechanical stimulation, demonstrating a versatile platform for bone tissue engineering applications.

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