Genotyping-by-Sequencing of Passion Fruit (Passiflora spp.) Generates Genomic Resources for Breeding and Systematics

Authors

• Joshua D. Anderson, University of Florida
• Ravena Ferreira Vidal, Universidade Estadual do Norte Fluminense
• Maria Brym, University of Florida
• Eric T. Stafne, Mississippi State UniversityFollow
• Marcio F. R. Resende, University of Florida
• Alexandre Pio Viana, Universidade Estadual do Norte Fluminense
• Alan H. Chambers, University of Florida

ORCID

Chambers: https://orcid.org/0000-0002-6765-0652

MSU Affiliation

Extension Service (MSUES); Coastal Research and Extension Center

Creation Date

2026-06-30

Abstract

The passion fruit genus (Passiflora) is comprised of more than 600 species with several species admired for their tropical fruit and ornate flowers. Passiflora edulis Sims is the most important species and includes yellow or purple fruit types. Major threats to commercial production include both abiotic and biotic stresses, and recent advances in passion fruit genomics are facilitating systematic plant improvement. Marker-assisted breeding of passionfruit enables parental selection, confirmation of hybrid parentage, correction of mis-identified accessions, and resolution of species assignments. Therefore, the purpose of this study was to develop single nucleotide polymorphism (SNP) markers to characterize 59 accessions from 21 species that included native and naturalized Passiflora species found in Florida, USA. Over 2 million SNPs were identified and 26,191 were used for downstream analyses. Phylogenetic, STRUCTURE, and Principal Component Analyses separated species and identified mislabeled accessions. Passiflora edulis accessions including yellow and purple-fruited types were grouped by fruit type. Eight species found in Florida including P. biflora Lam., P. foetida L., P. incarnata L., P. lutea L., P. mulitiflora L., P. pallens Poepp. ex Mast., P. sexflora Juss., and P. pallida L. were well-separated from other species. Sequencing four naturally-pollinated P. laurifolia L. seedlings demonstrated that this accession is self-compatible. The results from this study could aid the genetic improvement of passion fruit in the future.

Publication Date

5-26-2022

Publication Title

Genetic Resources and Crop Evolution

Publisher

Springer

First Page

2769

Last Page

2786

Recommended Citation

Anderson, J.D., Vidal, R.F., Brym, M. et al. Genotyping-by-sequencing of passion fruit (Passiflora spp.) generates genomic resources for breeding and systematics. Genet Resour Crop Evol 69, 2769–2786 (2022). https://doi.org/10.1007/s10722-022-01397-4