Human Immunoglobulin G Responses to Cimex lectularius L. Saliva

ORCID

Sheele: https://orcid.org/0000-0003-4503-8590; Goddard: https://orcid.org/0000-0002-2617-7141

MSU Affiliation

College of Agriculture and Life Sciences; Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology

Creation Date

2026-09-30

Abstract

AIMS: To investigate the immunoglobulin (Ig) G response after being fed upon by Cimex lectularius L. METHODS AND RESULTS: Participants were fed upon by three male C lectularius insects weekly for a month. Blood was obtained before the feeding and at the last feeding, which was used for immunoblots against bed bug salivary gland extract, with antihuman Immunoglobulin G (IgG) secondary antibodies. No consistent IgG changes developed in 11 humans serially fed upon by C lectularius. Two participants had new IgG responses to proteins at molecular weights of approximately 12-13 kDa, and one had an IgG response to a protein at approximately 40 kDa. At the last study visit, more intense IgG bands to proteins at molecular weights of 12-13 kDa had developed in 55% of participants (6/11) and at molecular weights of ≈30, ≈40 and ≈70 kDa in 45% (5/11) compared with the first study visit. Nitrophorin and apyrase were the most common C lectularius proteins identified with liquid chromatography-tandem mass spectrometry in both crushed bed bug salivary gland extract and post-bed bug feeding extract. CONCLUSIONS: Human participants did not have consistent IgG responses to crushed C lectularius salivary gland extract.

Keywords

Cimex lectularius, IgG, antibody, bed bug, cimicosis, immunoglobulin, saliva

Publication Date

6-9-2020

Publication Title

Parasite Immunology

Publisher

Wiley

Rights

© 2020 John Wiley & Sons Ltd

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Digital Object Identifier (DOI)

https://doi.org/10.1111/pim.12764