Artificial Fertilization for Amphibian Conservation: Current Knowledge and Future Considerations

ORCID

CVance: https://orcid.org/0000-0001-8466-6077

MSU Affiliation

College of Agriculture and Life Sciences; Department of Biochemistry and Molecular Cell Sciences

Creation Date

2026-10-30

Abstract

Amphibian populations in the wild are experiencing massive die-offs that have led to the extinction of an estimated 168 species in the last several decades. To address these declines, zoological institutions are playing an important role in establishing captive assurance colonies to protect species in imminent danger of extinction. Many of the threatened species recently placed into captivity are failing to reproduce before they expire, and maintaining founder populations is becoming a formidable challenge. Assisted reproductive technologies, such as hormone synchronization, gamete storage and artificial fertilization, are valuable tools for addressing reproductive failure of amphibians in captive facilities. Artificial fertilization has been commonly employed for over 60 years in several keystone laboratory species for basic studies in developmental biology and embryology. However, there are few instances of applied studies for the conservation of threatened or endangered amphibian species. In this review, we summarize valuable technological achievements in amphibian artificial fertilization, identify specific processes that need to be considered when developing artificial fertilization techniques for species conservation, and address future concerns that should be priorities for the next decade. © 2008 Elsevier Inc. All rights reserved.

Keywords

amphibian, artificial fertilization, egg, hormones, sperm

Publication Date

11-20-2008

Publication Title

Theriogenology

Publisher

Elsevier

First Page

214

Last Page

227

Rights

© 2008 Elsevier Inc. All rights reserved.

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