Theses and Dissertations

Issuing Body

Mississippi State University

Advisor

Elder, Steven H.

Committee Member

Butler, James Ryan

Committee Member

Liao, Jun

Committee Member

Williams, Lakiesha N.

Committee Member

Simpson, Chartrisa LaShan

Date of Degree

5-7-2016

Document Type

Dissertation - Open Access

Major

Biomedical Engineering

Degree Name

Doctor of Philosophy (Ph.D)

College

James Worth Bagley College of Engineering

Department

Department of Agricultural and Biological Engineering

Abstract

Articular cartilage provides an almost frictionless surface for the articulating ends of the bone. Cartilage functions to lubricate and transmit compressive forces resulting from joint loading and impact. If the cartilage is damaged, through traumatic injury or disease, it lacks the ability of self-repairing as the tissue lacks vascular system. If the injuries to articular cartilage are left untreated, they may progress to Osteoarthritis. Osteoarthritis, a degenerative disease, is one of the leading disabilities in the United States. Tissue engineering has the potential to regenerate healthy hyaline cartilage, which can alleviate pain and restore the functions of normal tissue. This study explores the production of engineered cartilage on top of composite calcium phosphate scaffold. The current research is related to a biphasic approach to cartilage tissue engineering — in which one layer supports to form subchondral bone (osteogenesis) and another supports cartilage formation (chondrogenesis). Chondrocyte and bone marrow-derived stem cell attachment to chitosan will be investigated for producing a bilayered construct for osteochondral repair. The main objectives of my research include the following: attachment and proliferation of human mesenchymal stem cells on chitosan calcium phosphate scaffolds, techniques to create a biphasic construct, the effect of coating chitosan calcium phosphate scaffolds with type I collagen and determining the ideal bead size for making chitosan calcium phosphate scaffolds.

URI

https://hdl.handle.net/11668/19216

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