Theses and Dissertations

Tianmin Li

Issuing Body

Mississippi State University

Advisor

Zhang, Li

Committee Member

Sukumaran, Anuraj

Committee Member

Cheng, Wen-Hsing

Committee Member

Kiess, Aaron S.

Date of Degree

11-25-2020

Original embargo terms

Complete embargo for 1 year

Document Type

Graduate Thesis - Open Access

Major

Food Safety

Degree Name

Master of Science

College

College of Agriculture and Life Sciences

Department

Department of Poultry Science

Abstract

Avian pathogenic Escherichia. coli (APEC) and Campylobacter are pathogenic threats to poultry and human health, respectively. In this study, the prevalence of these pathogens in Mississippi broilers and their antimicrobial resistance (AMR) properties were investigated, and a multidrug-resistant APEC strain (APEC-O2-MS1170) was further explored by whole-genome sequencing (WGS). The efficacy of in ovo injection of Lactobacillus in reducing the APEC in broilers was evaluated. Results revealed a high prevalence of APEC and Campylobacter in broilers and broiler products. A lot of isolates were resistant to antibiotics of different sorts. Moreover, the in ovo administration of Lactobacillus did not reduce the incidence of APEC. The WGS of APEC-O2-MS1170 revealed its detailed AMR and virulence properties and alerted a potential zoonotic risk. In conclusion, the Lactobacillus did not reduce the incidence of APEC in broilers, and the prevalence and AMR of APEC and Campylobacter are still challenges faced by the poultry industry.

URI

https://hdl.handle.net/11668/20868

Comments

Avian pathogenic Escherichia coli||Campylobacter||multidrug-resistant||virulence gene||whole-genome sequencing

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