Validation Study of a Novel Detection Kit for Rapid Detection and Quantification of Listeria Spp. in Food Samples

Author

Mengying Jiang

Issuing Body

Mississippi State University

Advisor

Kim, Taejo

Committee Member

Silva, Juan L.

Committee Member

Schilling, M. Wes

Date of Degree

8-17-2013

Document Type

Graduate Thesis - Open Access

Major

Food Science and Technology

Degree Name

Master of Science

College

College of Agriculture and Life Sciences

Department

Department of Food Science, Nutrition and Health Promotion

Abstract

A single tube detection kit was designed as a rapid, easy-to use and reliable test to detect Listeria spp.. Various food samples (vegetables and raw catfish fillets) were used in order to validate the performance of the detection kit. L. grayi was detected in one ready-to-eat (RTE) vegetables with the detection kit while no Listeria spp. was detected using the modified FDA-BAM method. In addition, both the detection kit and modified FDA-BAM method indicated that twelve catfish fillets were Listeria positive. The detection kit had 100% sensitivity and specificity in less detection time (24 h) than the modified FDA-BAM method (60% specificity, >72 h). There was no difference (P<0.05) between the kit and the modified FDA-BAM method on MPN for Listeria spp.

URI

https://hdl.handle.net/11668/20185

Recommended Citation

Jiang, Mengying, "Validation Study of a Novel Detection Kit for Rapid Detection and Quantification of Listeria Spp. in Food Samples" (2013). Theses and Dissertations. 4943.
https://scholarsjunction.msstate.edu/td/4943